Thursday, June 29th, 2017

 

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Title:
Apoptosis of Hormone-Dependent Prostate Cancer LNCaP Cells Induced by p21 Small Activating RNA in Combination with PC-3
Authors:  Zhenxing Wang, M.B., Hua Chen, M.B., Shaohua Shi, M.B., Yongwen Ma, M.B., Jun Yang, M.B., Xiaojun Hao, M.B., Pingping Sun, M.B., Huiying Wang, M.B., Xiaotong Wu, M.B., and Xiaolong Xing, M.B.
  Objective: To evaluate the effects of p21 small activating RNA (saRNA) in combination with PC-3 on the apoptosis of LNCaP cells.
Study Design:
LNCaP cells were collected 24 hours after the addition of PC-3 and 72 hours after being transfected with p21 saRNA. The MTT assay was used to determine the inhibition rate of cell viability. After Annexin-V-PI staining, flow cytometry was used to analyze the effect on apoptosis and cell cycles. Western blot was used to detect the changes of p21 protein expression.
Results:
p21 saRNA in combination with PC-3 significantly inhibited the viability of LNCaP cells, promoted apoptosis, and retarded the cell cycle to phase G0/G1 as compared with the results of p21 saRNA transfection or PC-3 for treatment alone. It was demonstrated by Western blot that this combination facilitated apoptosis and retarded cell cycle by activating the p21 network.
Conclusion:
p21 saRNA in combination with PC-3 can be used to induce LNCaP cell apoptosis and may become a tool for treating hormone-independent prostate cancer.
Keywords:  LNCaP, p21, PC-3, prostate cancer, RNA activation
   
   
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