Monday, November 19th, 2018

 

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Title:
Immunohistochemical Features of the Pseudomyxoma Peritonei Microenvironment: An Opportunity for Clinicians
Authors:  Fabio Grizzi, Ph.D., Ferdinando Carlo Maria Cananzi, M.D., Serena Battista, M.D., Tatiana Brambilla, M.D., Dorina Qehajaj, B.Sc., Maurizio Chiriva-Internati, Ph.D., Uberto Fumagalli Romario, M.D., Vittorio Quagliuolo, M.D., and Pietro Francesco Bagnoli, M.D.
  Objective: To provide new details on the pseudomyxoma peritonei (PMP) microenvironment and discuss its potential role on the clinical behavior of this tumor.
Study Design:
In the present study, in addition to routine histology, immunostains for CD68, CD3, CD20, Ki-67, CD34, LYVE-1, and the tumor-associated antigens (TAAs) pituitary-tumor transforming gene 1 (PTTG1) and squamous cell carcinoma antigen 1 (SCCA1) were explored. Fourteen consecutive patients who underwent cytoreductive surgery with hyperthermic intraoperative peritoneal chemotherapy were included in the study.
Results:
We found the presence of variable amounts and pattern distributions of CD68+ cells, CD3+ T-cells, and CD20+ B-cells in the PMP microenvironment. CD3+ lymphocytes were grouped in clusters in 7 out of 14 (50%) PMPs, while only 4 out of 14 (29%) had dispersed CD20+ lymphocytes. CD68+ macrophages were always found dispersed throughout the PMP microenvironment. PMPs have been also found highly vascularized by blood vessels when compared to LYVE-1+ lymphatic vessels, and variably proliferative as shown by different Ki- 67+ cell densities. Additionally, PMPs were immunopositive for PTTG1 and SCCA1, 2 TAAs previously associated with the progression and recurrence of various human malignancies.
Conclusion:
Our findings highlight unknown features about PMP microenvironment organization and represent a basis for additional studies including a large cohort of patients to reveal helpful information on the clinical behavior of this tumor.
Keywords:  biomarkers, immunity, pituitary-tumor transforming gene 1, pseudomyxoma peritonei, squamous cell carcinoma antigen 1
   
   
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