Thursday, February 21st, 2019


Multiple Sections per Slide for Immunohistochemistry: A Cost-Effective Alternative in Resource-Limited Settings
Authors:  Andrey Bychkov, M.D., Ph.D., and Deepali Jain, M.D., DNB
  To the Editors: Immunohistochemistry (IHC) is the most common ancillary technique in pathology practice. It also serves as a driving tool for research in surgical pathology, especially in resource-limited settings where advanced molecular approaches are not widely available. Introduction of automated immunostainers significantly saves labor and costs and reduces turnaround time for the full workflow of IHC procedures. However, most automated stainers require a particular volume of reagent to be dispensed at each step of a protocol for the capillary action or air pressure, which is necessary to distribute the reagent across the entire slide.1 This amount is 200 μL in default settings, and only some more advanced and expensive stainers offer an option of adjustable dispensing volume.
Keywords:  automated immunostaining; automation, laboratory; clinical laboratory techniques; histological labeling; immunohistochemistry; staining; staining and labeling
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