Wednesday, July 17th, 2019


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Effect of Yiqi Bushen Recipe on Expression of Notch1 and Jagged1 in Tumor Cells of Mouse Gastric Cancer Model
Authors:  Yun-xia Liu, Ph.D., Ye-feng Xu, M.M., Yong-wei Yao, M.M., Shen-jun Jiang, M.M., Na Wang, M.M., and Meng-yun Li, M.M.
  Objective: To explore the molecular mechanism of multiple targets of Yiqi Bushen Recipe (YBR) in resisting gastric cancer metastasis.
Study Design:
A cancer stem cell (CSC)–enriched, tumor-bearing nude mouse model was established by using luciferase-labeled human gastric cancer cell line SGC-7901-luc. The experimental mice included 5 groups: negative control (group 1), model control (group 2), 5-fluorouracil injection (5-FU group 3), YBR (group 4), and YBR+5-FU (group 5). The drug intervention time was 6 weeks (groups 3, 4, and 5). The protein expres-sions of Notch homolog 1 (Notch1) and Jagged1 (Jag1) were detected by western blot. The mRNA expressions of Notch1 and Jag1 were detected by RT-PCR.
The Notch1 and Jag1 protein concentrations in the groups 4 and 5 were lower than those in group 2 (p<0.05). The level of Notch1 and Jag1 mRNA was lowest in group 5, followed by group 4, and their concentrations were significantly different from group 2 and group 3 (p<0.05).
The mechanism of YBR against gastric cancer (GC) metastasis may be related to the downregulation of the protein and mRNA expression of Notch1 and Jag1. It is beneficial for patients with GC to take YBR.
Keywords:  cancer stem cells, gastric cancer, Jagged-1, Jagged1 protein, molecular mechanism, molecular mechanisms of pharmacological action, neoplastic stem cells, Notch1 protein, Notch1 receptor, Notch1 and Jagged1 signaling pathway, signal pathways, tumor metastasis, Yiqi Bushen recipe
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