Thursday, August 22nd, 2019


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Effect of SDF-1/CXCR4 Pathway on Bone Marrow Mesenchymal Stem Cells Under Direct Current Microelectric Field
Authors:  Hao Wu, M.D., Yuxuan Gao, M.D., Na Liu, M.D., Wei Zhang, M.D., Haigang Shi, M.D., and Hongbo Li, M.D.
  Objective: To observe correlation of direct current electric field (DCEF) with bone marrow mesenchymal stem cell (BMSC) migration and proliferation through isolated culture of BMSCs of GFP (expressing green fluorescent protein) rats.
Study Design:
GFP-BMSCs were cultured in an isolated way, and flow cytometry was used for analysis and identification. Transwell cell migration experiment was applied to screen DCEF action time most suitable for cell migration and function of SDF-1/CXCR4 under DCEF. The MTT method was used to determine cell proliferation curve of BMSCs for the group with DCEF and the group without DCEF.
The third generation of GFP-BMSCs analyzed and identified by flow cytometry was gained. The number of migrating BMSCs reached the maximum when the action time of 200 mV/mm DCEF was 4 hours (p<0.05). After SDF-1 was added in the lower chamber, cell migration quantity increased, while cell migration quantity decreased after the blocking agent was added (p<0.05). After DCEF was loaded, BMSC proliferation reached the peak on the third day, higher than the peak value of the control group (p<0.05).
The effect of SDF-1/CXCR4 pathway on BMSCs may be closely related to DCEF. The application of DCEF can facilitate BMSC proliferation.
Keywords:  bone marrow mesenchymal stem cells; cell migration; CXCL12 chemokine; DCEF; differentiation; direct current electric field; mesenchymal stem cells; SDF-1; SDF-1/CXCR-4; stromal cell-derived factor 1
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